• Product NameMEK1/2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total MEK1/2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.235-239(L-Q-G-T-H) derived from Human MEK1/2.
• Target NameMEK1/2
• Alternative Names    MEK1; MKK1; MAPKK1; PRKMK1;
• Accession No. Swiss-Prot: Q02750
  NCBI Protein: NP_002746.1
• UniprotQ02750
• Gene ID5604;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 45kd
  Western blotting: 1:500~1:1000
  

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Seger R., Seger D., Lozeman F.J., Ahn N.G., Graves L.M., Campbell J.S., Ericsson L., Harrylock M., Jensen A.M., Krebs E.G.J. Biol. Chem. 267:25628-25631(1992)
Mukherjee S., Keitany G., Li Y., Wang Y., Ball H.L., Goldsmith E.J., Orth K.Science 312:1211-1214(2006)

• Western blot analysis of extract from Hela and JK cells using MEK1/2 Antibody #21428

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting