• Product NameOct-1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total Oct-1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.270~274(T-P-K-R-I) derived from Human Oct-1.
• Target NameOct-1
• Alternative Names    1-Oct; OTF1; oct-1B
• Accession No. Swiss-Prot: P14859
  NCBI Protein: NP_001185712.1
• UniprotP14859
• Gene ID5451;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20℃ for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 90kd
  Western blotting: 1:500~1:1000
  Immunofluorescence：1:100~1:200
   

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

Prefontaine G.G., Walther R., Giffin W. J. Biol. Chem. 274:26713-26719(1999)
Mahajan S.S., Wilson A.C. Mol. Cell. Biol. 20:919-928(2000)

• Western blot analysis of extracts from JK, HepG2 and 293 cells using Oct-1 Antibody #21674.
• Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using Oct-1 Antibody #21674.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting