• Product NameCHML Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CHML protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CHML.
• Target NameCHML
• Alternative Names    Rab proteins geranylgeranyltransferase component A 2; Rab escort protein 2; REP-2; Choroideraemia-like protein;
• Accession No. Swiss-Prot: P26374
  NCBI Gene ID: 1122
• UniprotP26374
• Gene ID1122;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.

Cremers F.P.M., Hum. Mol. Genet. 1:71-75(1992).
Kasper G., Gene 295:27-32(2002).
Ota T. Nat. Genet. 36:40-45(2004)

• Western blot analysis of extracts from COLO cells, using CHML antibody #34948.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CHML antibody #34948.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting