• Product NameIGFBP-3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total IGFBP-3 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.185~189(R-Y-K-V-D) derived from Human IGFBP-3.
• Target NameIGFBP-3
• Alternative Names    IBP3; BP-53;
• Accession No. Swiss-Prot: P17936
  NCBI Protein: NP_001013416.1
• UniprotP17936
• Gene ID3486;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 24-41kd
  Western blotting: 1:500~1:1000
   

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Roghani M., Segovia B., Whitechurch O., Binoux M.Growth Regul. 1:125-130(1991)
Ikonen M., Liu B., Hashimoto Y., Ma L. Cohen P.Proc. Natl. Acad. Sci. U.S.A. 100:13042-13047(2003)
Zhou W., Ross M.M., Tessitore A. J. Proteome Res. 8:5523-5531(2009)

• Western blot analysis of extracts from various cells using IGFBP-3 Antibody #21671.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting