• Product NameBeclin-1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Beclin-1 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.69-73( F-I-E-T-P) derived from Human Beclin-1.
• Target NameBeclin-1
• Alternative Names    ATG6; VPS30;
• Accession No. Swiss-Prot: Q14457
  NCBI Protein: NP_003757.1
• UniprotQ14457
• Gene ID8678;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 60kd
  Western blotting: 1:500~1:1000
  

Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Sagona A.P., Nezis I.P., Pedersen N.M., Liestol K., Poulton J., Rusten T.E., Skotheim R.I., Raiborg C., Stenmark H.Nat. Cell Biol. 12:362-371(2010)
Sun Q., Fan W., Chen K., Ding X., Chen S., Zhong Q.Proc. Natl. Acad. Sci. U.S.A. 105:19211-19216(2008)

• Western blot analysis of extracts from Raji and K562 cells using Beclin-1 Antibody #21582.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting