• Product NameNiban(Phospho-Ser602) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Niban only when phosphorylated at serine 602.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 602 (R-A-S(p)-A-I) derived from Human Niban.
• Target NameNiban
• ModificationPhospho
• Alternative Names    FAM129A
• Accession No. Swiss-Prot: Q9BZQ8
  NCBI Protein: NP_443198.1
• UniprotQ9BZQ8
• Gene ID116496;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 150kd
  Western blotting: 1:500~1:1000

Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response

Vill

• Western blot analysis in vitro kinase assays were performed by mixing purified bacterially expressed WT His–Niban or the His–Niban S602A with or without purified active AKT1 using Niban (Phospho-Ser602) Antibody #11578.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting