• Product NameACTIN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB,ELISA
• Species ReactivityPlants
• SpecificityThe antibody detects endogenous levels of total ACTIN protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide of Arabidopsis thaliana actin
• Target NameACTIN
• Alternative Names    ACTB
• Accession No. Swiss-Prot#: P53497
  NCBI Gene ID: 823805
  Gene Accssion: NP_190236.1
• UniprotP53497
• Gene ID823805;
• Calculated MW 42kDa
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

• Gel: 8%SDS-PAGELysate: 40 μgLane 1-2: Corn leaves lysate, Rice leaves lysatePrimary antibody: ACTIN Antibody at dilution 1/1000Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 minutes

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting