• Product NameGFP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• SpecificityThe antibody detects transfected proteins contanining GFP tag.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameGFP
• Alternative Names    GFP-like chromoprotein
• Accession No. Gene Accssion: P42212
• SDS-PAGE MW 27kd
• Concentration 1.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:10000

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

• Gel: 8%SDS-PAGE
  Lysate: 20ug GFP transfected 293T cell
  Primary antibody: 1/800 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Gel: 8%SDS-PAGE
  Lysates: 20ug GFP transfected 293T cell per lane
  Primary antibody: at various dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting