• Product Nameribosome binding protein 1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 750 and 957 of ribosome binding protein 1
• Target Nameribosome binding protein 1
• Accession No. Swiss-Prot:Q9P2E9
  Gene ID:6238
• UniprotQ9P2E9
• Gene ID6238;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 40% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 109kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Hep G2
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1 antibody at 1: 500 dilution.
• Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2500.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting