• Product NameSUFU Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total SUFU protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human Suppressor of fused homolog (Drosophila)
• Target NameSUFU
• Alternative Names    SUFUH; SUFUXL; PRO1280
• Accession No. Swiss-Prot#: Q9UMX1
  NCBI Gene ID: 51684
  Gene Accssion: BC013291
• UniprotQ9UMX1
• Gene ID51684;
• SDS-PAGE MW 54kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:15-1:50

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

• Gel: 10%SDS-PAGE
  Lysate: 50ug Jurkat cell
  Primary antibody: 1/100 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #35993 at dilution 1/10.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting