• Product NameAFAP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total AFAP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human Actin filament associated protein 1
• Target NameAFAP1
• Alternative Names    AFAP; AFAP110; AFAP-110
• Accession No. Swiss-Prot#: Q8N556
  NCBI Gene ID: 60312
  Gene Accssion: BC032777
• UniprotQ8N556
• Gene ID60312;
• SDS-PAGE MW 90kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 8%SDS-PAGE
  Lysate: 40ug A172 cell
  Primary antibody: 1/134 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #36056 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting