• Product NameAGA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total AGA protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human Aspartylglucosaminidase
• Target NameAGA
• Alternative Names    GA; AGU; ASRG
• Accession No. Swiss-Prot#: P20933
  NCBI Gene ID: 175
  Gene Accssion: BC012392
• UniprotP20933
• Gene ID175;
• SDS-PAGE MW 37kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:15-1:50

Aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme. Alternatively spliced transcript variants have been identified.?

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Mouse kidney tissue, human testis and thyroid cancer tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/100 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #36057 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting