• Product NameNIF3L1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NIF3L1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human NIF3 NGG1 interacting factor 3-like 1 (S. cerevisiae)
• Target NameNIF3L1
• Alternative Names    CALS-7; MDS015; ALS2CR1
• Accession No. Swiss-Prot#: Q9GZT8
  NCBI Gene ID: 60491
  Gene Accssion: BC007654
• UniprotQ9GZT8
• Gene ID60491;
• SDS-PAGE MW 42kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

NIF3-like protein 1?is a?protein?that in humans is encoded by the?NIF3L1?gene. NIF3L1 has been shown to interact with COPS2. Expression analysis on cell lines revealed strong overexpression in a spermatogonia-derived cell line and a teratocarcinoma cell line. A human NIF3L1/GFP fusion protein showed localization of the protein in the cytoplasm.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Jurkat cell and human fetal kidney tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36088 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting