• Product NameATG5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ATG5 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameATG5
• Alternative Names    ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Accession No. Swiss-Prot#: Q9H1Y0
  NCBI Gene ID: 9474
  Gene Accssion: BC002699
• UniprotQ9H1Y0
• Gene ID9474;
• SDS-PAGE MW 32kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

Autophagy protein 5?is a?protein?that in humans is encoded by the?ATG5?gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

• Gel: 12%SDS-PAGE
  Lysates (from left to right): NIH/3T3 and A172 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/475 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36116 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting