• Product NameARHGAP15 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ARHGAP15 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human Rho GTPase activating protein 15
• Target NameARHGAP15
• Alternative Names    BM046
• Accession No. Swiss-Prot#: Q53QZ3
  NCBI Gene ID: 55843
  Gene Accssion: BC016701
• UniprotQ53QZ3
• Gene ID55843;
• SDS-PAGE MW 55kd
• Concentration 2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

RHO GTPases regulate diverse biologic processes, and their activity is regulated by RHO GTPase-activating proteins (GAPs), such as ARHGAP15. GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Jurkat and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 2 minutes
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36131 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting