• Product NameINTS10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total INTS10 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human integrator complex subunit 10
• Target NameINTS10
• Alternative Names    INT10; C8orf35
• Accession No. Swiss-Prot#: Q9NVR2
  NCBI Gene ID: 55174
  Gene Accssion: BC001915
• UniprotQ9NVR2
• Gene ID55174;
• SDS-PAGE MW 82kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1. INTS10 (integrator complex subunit 10) is also known as INT10 and is a 710 amino acid protein that is localized to the nucleus. INTS10 is a component of the integrator complex and, as such, is thought to aid in the regulation of 3¡¯-end processing of spliceosomal U1 and U2 snRNAs.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Hela and hepG2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/475 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36159 at dilution 1/50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting