• Product NameASAH2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ASAH2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
• Target NameASAH2
• Alternative Names    HNAC1; BCDase; LCDase; NCDase; N-CDase
• Accession No. Swiss-Prot#: Q9NR71
  NCBI Gene ID: 56624
  Gene Accssion: BC107105
• UniprotQ9NR71
• Gene ID56624;
• SDS-PAGE MW 86kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Human fetal kidney tissue and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/240 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using #36240 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting