• Product NameBCAT2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total BCAT2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human branched chain amino-acid transaminase 2, mitochondrial
• Target NameBCAT2
• Alternative Names    BCAM; BCT2; PP18; BCATM
• Accession No. Swiss-Prot#: O15382
  NCBI Gene ID: 587
  Gene Accssion: BC001900
• UniprotO15382
• Gene ID587;
• SDS-PAGE MW 44kd
• Concentration 2.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:100-1:300

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.

• Gel: 12%SDS-PAGE
  Lysates (from left to right): Human placenta tissue and NIH/3T3 cell, Raji and hepG2 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/312.5 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36277 at dilution 1/70.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting