• Product NameCENPH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CENPH protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameCENPH
• Alternative Names    CENP-H; ICEN35;Centromere protein H
• Accession No. Swiss-Prot#: Q9H3R5
  NCBI Gene ID: 64946
  Gene Accssion: BC012024
• UniprotQ9H3R5
• Gene ID64946;
• SDS-PAGE MW 28kd
• Concentration 1mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C.?

• Gel: 8%SDS-PAGE
  Lysate: 40ug Hela cell
  Primary antibody: 1/250 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 second

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting