• Product NameDEPDC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DEPDC1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human DEP domain containing 1
• Target NameDEPDC1
• Alternative Names    DEP.8; SDP35; DEPDC1A; DEPDC1-V2
• Accession No. Swiss-Prot#: Q5TB30
  NCBI Gene ID: 55635
  Gene Accssion: BC065304/Q5TB30
• UniprotQ5TB30
• Gene ID55635;
• SDS-PAGE MW 93kd
• Concentration 0.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

DEP domain containing 1?is a?protein?in humans that is encoded by the DEPDC1?gene. May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): A172, hela, hepg2 and 293T cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/150 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 1 minute

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting