• Product NameMYL12B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MYL12B protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFull length fusion protein
• Target NameMYL12B
• Alternative Names    MLC-B; MRLC2
• Accession No. Swiss-Prot#: O14950
  NCBI Gene ID: 103910
  Gene Accssion: BC004994
• UniprotO14950
• Gene ID103910;10627;
• SDS-PAGE MW 20kd
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse skeletal muscle, heart and bladder tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/450 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using #36467 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting