• Product NameHMGCL Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total HMGCL protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human 3-hydroxymethyl-3-methylglutaryl-CoA lyase
• Target NameHMGCL
• Alternative Names    HL
• Accession No. Swiss-Prot#: P35914
  NCBI Gene ID: 3155
  Gene Accssion: BC010570
• UniprotP35914
• Gene ID3155;
• SDS-PAGE MW 34kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): SKOV3 cell, mouse heart and brain tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/150 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36521 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting