• Product NameHACL1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HACL1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human 2-hydroxyacyl-CoA lyase 1
• Target NameHACL1
• Alternative Names    HPCL; HPCL2; PHYH2; 2-HPCL
• Accession No. Swiss-Prot#: Q9UJ83
  NCBI Gene ID: 26061
  Gene Accssion: BC001627
• UniprotQ9UJ83
• Gene ID26061;
• SDS-PAGE MW 64kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:50-1:200

HACL1 (2-hydroxyacyl-CoA lyase 1) is also known as HPCL or 2-HPCL (2-hydroxyphytanoyl-CoA lyase) and is a 578 amino acid protein. HACL1 is abundantly expressed in liver, and is also expressed in kidney, heart and skeletal muscle, where it is localized to peroxisomes. HACL1 functions in lipid metabolism as well as fatty acid metabolism and is able to form homotetramers.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Human testis tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/200 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 30 seconds
• Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using #36525 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting