• Product NameKARS Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total KARS protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human lysyl-tRNA synthetase
• Target NameKARS
• Alternative Names    KRS; KARS1; KARS2; CMTRIB; DFNB89
• Accession No. Swiss-Prot#: Q15046
  NCBI Gene ID: 3735
  Gene Accssion: BC004132
• UniprotQ15046
• Gene ID3735;
• SDS-PAGE MW 68kd
• Concentration 2.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:100-1:300

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): K562, A549, HT-29, 293T, Hela and Jurkat cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #36565 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting