• Product NamePCK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PCK2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human phosphoenolpyruvate carboxykinase 2
• Target NamePCK2
• Alternative Names    PEPCK; PEPCK2; PEPCK-M
• Accession No. Swiss-Prot#: Q16822
  NCBI Gene ID: 5106
  Gene Accssion: BC001454
• UniprotQ16822
• Gene ID5106;
• SDS-PAGE MW 71kd
• Concentration 2.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

• Gel: 6%SDS-PAGE
  Lysates (from left to right): 293T, Jurkat and Hela cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/600 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 minutes
• Immunohistochemical analysis of paraffin-embedded Human brain tissue using #36616 at dilution 1/60.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting