• Product NamePPP2CA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PPP2CA protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the N terminal of human protein phosphatase 2, catalytic subunit, alpha isozyme
• Target NamePPP2CA
• Alternative Names    RP-C; PP2Ac; PP2CA; PP2Calpha
• Accession No. Swiss-Prot#: P67775
  NCBI Gene ID: 5515
  Gene Accssion: BC002657/P67775
• UniprotP67775
• Gene ID5515;
• SDS-PAGE MW 36kd
• Concentration 1.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000-1:5000
  Immunohistochemistry: 1:25-1:100

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

• Gel: 10%SDS-PAGE
  Lysates (from left to right): Mouse brain tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/450 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36699 at dilution 1/35.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting