• Product NamePDP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PDP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human pyruvate dehyrogenase phosphatase catalytic subunit 1
• Target NamePDP1
• Alternative Names    PDH; PDP; PDPC; PPM2C
• Accession No. Swiss-Prot#: Q9P0J1
  NCBI Gene ID: 54704
  Gene Accssion: BC047619/Q9P0J1
• UniprotQ9P0J1
• Gene ID54704;
• SDS-PAGE MW 61kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1:25-1:100

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits.?

• Gel: 6%SDS-PAGE
  Lysates (from left to right): Human bladder carcinoma tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/300 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36700 at dilution 1/25.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting