• Product NamePSMC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total PSMC1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human proteasome (prosome, macropain) 26S subunit, ATPase, 1
• Target NamePSMC1
• Alternative Names    S4; p56; P26S4
• Accession No. Swiss-Prot#: P62191
  NCBI Gene ID: 5700
  Gene Accssion: BC016368/P62191
• UniprotP62191
• Gene ID5700;
• SDS-PAGE MW 49kd
• Concentration 2.2mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:50-1:200

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): A549, hela and NIH/3T3 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/500 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 20 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36704 at dilution 1/40.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting