• Product NamePYGM Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total PYGM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to residues near the C terminal of human phosphorylase, glycogen, muscle
• Target NamePYGM
• Alternative Names    Myophosphorylase; phosphorylase; glycogen;muscle
• Accession No. Swiss-Prot#: P11217
  NCBI Gene ID: 5837
  Gene Accssion: BC126392/P11217
• UniprotP11217
• Gene ID5837;
• SDS-PAGE MW 97kd
• Concentration 1.6mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:30-1:150

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Mouse heart tissue
  Amount of lysate: 40ug per lane
  Primary antibody: 1/400 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 40 seconds
• Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #36710 at dilution 1/30.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting