• Product NameRBBP5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total RBBP5 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human retinoblastoma binding protein 5
• Target NameRBBP5
• Alternative Names    RBQ3; SWD1
• Accession No. Swiss-Prot#: Q15291
  NCBI Gene ID: 5929
  Gene Accssion: BC053856/Q15291
• UniprotQ15291
• Gene ID5929;
• SDS-PAGE MW 59kd
• Concentration 1.3mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.?

• Gel: 8%SDS-PAGE
  Lysates (from left to right): Hela, hepg2 and HT-29 cell
  Amount of lysate: 40ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 10 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting