• Product NameADO Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ADO protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide corresponding to a region derived from internal residues of human 2-aminoethanethiol dioxygenase
• Target NameADO
• Alternative Names    C10orf22;FLJ14547
• Accession No. Swiss-Prot#: Q96SZ5
  NCBI Gene ID: 84890
  Gene Accssion: NP_116193
• UniprotQ96SZ5
• Gene ID84890;
• SDS-PAGE MW 30kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1:25-1:100

Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine.

• Gel: 12%SDS-PAGE
  Lysates (from left to right): Mouse testis tissue
  Amount of lysate: 30ug per lane
  Primary antibody: 1/350 dilution
  Secondary antibody dilution: 1/8000
  Exposure time: 5 minutes
• Immunohistochemical analysis of paraffin-embedded Human renal cancer tissue using #36728 at dilution 1/20.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting