• Product NameNestin Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide corresponding to a sequence within amino acids 1392-1621 of human NES (NP_006608.1).
• Alternative Names    NES;Nbla00170;nestin;Nestin
• Accession No. Uniprot:P48681
  GeneID:10763
• UniprotP48681
• Gene ID10763
• Calculated MW 177 kDa
• SDS-PAGE MW 150/177 kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:1000;
  IHC 1:50 - 1:200;
  IF 1:50 - 1:200

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.

• Western blot analysis of lysates from Neuro-2a cells, using Nestin Polyclonal Antibody at 1:1000 dilution.
• Immunohistochemistry analysis of paraffin-embedded Mouse brain using Nestin Polyclonal Antibody at dilution of 100 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Rat brain using Nestin Polyclonal Antibody at dilution of 100 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
• Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Nestin Polyclonal Antibody at a dilution of 1:1000 (40x lens).
• Immunofluorescence analysis of Neuro-2a cells using Nestin Polyclonal Antibody at dilution of 1:50 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting