• Product NameNestin Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IF,IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant fusion protein of human Nestin (NP_006608.1).
• Alternative Names    NES;Nbla00170;nestin;Nestin
• Accession No. Uniprot:P48681
  GeneID:10763
• UniprotP48681
• Gene ID10763
• Calculated MW 177kDa
• SDS-PAGE MW 207KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:1000
  IF 1:50 - 1:100
  IHC 1:50 - 1:200

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.

• Western blot analysis of extracts of Rat testis, using Nestin antibody.
• Immunofluorescence analysis of U-251MG cells using Nestin antibody.
• Immunohistochemistry of paraffin-embedded Rat brain using Nestin antibody at dilution of 100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting