• Product NameJMJD3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB
• Species ReactivityHu Ms Rt
• SpecificityThis antibody is specific for JMJD3 and will not recognize other JMJD proteins.
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 16 amino acid peptide from near the amino terminus of human JMJD3.
• Target NameJMJD3
• Alternative Names    Jumonji domain-containing protein 3, Lysine-specific demethylase 6B, KDM6B
• Accession No. Swiss-Prot:O15054
  Gene ID:23135
• UniprotO15054
• Gene ID23135;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.

• Western blot analysis of JMJD3 in K562 cell lysate with JMJD3 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting